Science

The most crucial features of the γH2AX biomarker

• specificity of  the γH2AX for DNA double-stranded breaks (DSBs)
• insuperable sensitivity, as it can detect one double-stranded break in the nucleus of a cell quantification of the number of DNA double-stranded breaks per nucleus
• repeatability and reproducibility

over 60 clinical trials have utilised the γH2AX biomarker worldwide 

Selected bibliography 

E.P.Rogakou, D.R.Pilch, A.H.Orr, V.S.Ivanova, and W.M.Bonner. (1998). DNA double-strand breaks induce histone H2AX phosphorylation at serine-139. J. Biol. Chem. 273, 5858-5868.

E.P.Rogakou, C.Boon, C.Redon, W.M.Bonner. (1999) Megabase Chromatin Domains involved in DNA double strand breaks in vivo. J. Cell Biol. 146, 905-916